Molecular study of sapovirus in acute gastroenteritis in children: a cross-sectional study

Name	World Continuing Education Alliance
Activity Title	Molecular study of sapovirus in acute gastroenteritis in children: a cross-sectional study
Details	Sapovirus is a single strand non enveloped RNA virus that belongs to the. The laboratory methods for detection of sapovirus include enzyme-linked immunosorbent assay, electron microscopy, reverse transcription-polymerase chain reaction (RT-PCR) and next-generation sequencing. Diarrhea represents the second common cause of death in children and is associated with about two million deaths around the world. There are data available about sapovirus infection in different countries such as Perun Objectives Course Objective Sapovirus has emerged as a viral cause of acute gastroenteritis. However, there are insufficient data about the presence of this virus among children with acute gastroenteritis. The present study aimed to evaluate the presence of sapovirus in children with acute gastroenteritis by reverse transcriptase-polymerase chain reaction (RT-PCR).. A cross-sectional study enrolled 100 children patients with acute gastroenteritis from outpatient clinics with excluded bacterial pathogens and parasitic infestation. A stool sample was collected from each child for laboratory examination. Each stool sample was subjected to study by direct microscopic examination, study for rotavirus by enzyme-linked immunoassay (ELISA) and the remaining sample was subjected to RNA extraction and RT- PCR for sapovirus.. The most frequently detected virus was rotavirus by ELISA (25%). RT-PCR detected sapovirus in 7% of the stool samples. The children with sapovirus were all from rural regions and presented mainly dur...
Competence	Paediatrics and Child Health
Start Date	<span class="not-set">(not set)</span>
End Date	<span class="not-set">(not set)</span>
Event Time	08:15 AM
Location	World Continuing Education Alliance eLearning System
Cost (UGX)	0
CPD Points	1