Andrographolide, isolated from Andrographis paniculata, induces apoptosis in monocytic leukemia and multiple myeloma cells via augmentation of reactive oxygen species production

Name	World Continuing Education Alliance
Activity Title	Andrographolide, isolated from Andrographis paniculata, induces apoptosis in monocytic leukemia and multiple myeloma cells via augmentation of reactive oxygen species production
Details	Many plant-derived products possess a potential for use in chemotherapy. For example, vincristine (VCR) and vinblastine—two natural alkaloids isolated from. Andro has been shown to have anti-tumor activities against solid and hematopoietic tumor cell lines, established from colon-, gastric-, liver-, breast-, and prostatic cancers, leukemia, and lymphoma (. THP-1 (RRID:CVCL_0006) is a permanent human monocytic cell line derived from an acute monocytic leukemia patient (. H929 (aka “NCI-H929”) (RRID:CVCL_1600) is a permanent human IgA-kappa-producing multiple myeloma cell line (. In the present study, we investigated the viability-reducing effects and their mechanisms of Andro on both THP-1 and H929 cells. The effects of Andro were compared with those of the common anti-cancer drugs VCR and Ara-C (aka cytarabine). VCR and Ara-C are widely used as chemotherapeutic agents against soft tissue tumors and hematopoietic tumors including acute leukemia, lymphoma, and multiple myeloma (n ... Objectives Course Objective : Andrographolide (Andro) is a diterpenoid component of the plant. : We studied the effects of Andro on the viability of the human leukemia monocytic cell line THP-1 and the human multiple myeloma cell line H929. Andro was compared with cytosine arabinoside (Ara-C) and vincristine (VCR), which are well-established therapeutics against hematopoietic tumors.. : Andro reduced the viability of THP-1 and H929 in a dose-dependent manner. H929 viability was highly susceptible to Andro, although only slightly susceptible to Ara-C. The agents Andro, Ara-C, and VCR each induced apoptosis, as shown by cellular shrinkage, DNA fragmentation, and increases in annexin V-binding, caspase-3/7 activity, reactive oxygen species (ROS) production, and mitochondrial membrane depolarization.
Competence	General Medicine
Start Date	<span class="not-set">(not set)</span>
End Date	<span class="not-set">(not set)</span>
Event Time	06:00 PM
Location	World Continuing Education Alliance eLearning System
Cost (UGX)	0
CPD Points	1